HUMAN PAPILLOMA VIRUS GENOTYPING IN FRESH HEAD AND NECK TUMORS - OUR FIRST EXPERIENCE

Hpv 16 positive head and neck cancer.

Conținutul

    Department of Ophthalmology, Grigore T.

    helmintox chez la femme enceinte cancer la ficat in metastaza

    E-mail: moc. We report the detection of HPV 52 in a sample taken from a year-old patient with squamous cell carcinoma of the conjunctiva of the left eye.

    The method used for the detection of HPV was real time polymerase chain reaction.

    hpv 16 positive head and neck cancer

    The evolution was favorable after surgical removal of the tumor and the patient was explained that long-term follow-up is essential to avoid recurrence. Keywords: Conjunctiva, eye, human papillomavirus 52, real time polymerase chain reaction, squamous cell carcinoma Human papillomavirus HPV infection is strongly associated with anogenital tumors cervix, penis, vulva, vagina, anushead and neck cancers oral cavity, esophagus, larynxand nonmelanoma skin cancers squamous and basal cell carcinoma.

    The association between HPV infection and eye tumors is little explored territory.

    Aim: To implement the HPV genotyping method of fresh head and neck cancer samples in our laboratory. More than half of the patients recognized being chronic alcohol and tobacco users. Conclusions: The genotyping method was validated according to kit instructions.

    Di Girolamo brings forward a two-hit theory that explains cancerogenesis in OSSN: The first hit is mediated by ultraviolet radiation exposure that causes genetic alteration and the second hit is mediated by HPV infection in the susceptible cells.

    Case Report We present a unique case of squamous cell carcinoma of the conjunctiva examined and treated in June The year-old patient presented at the Ophthalmology Department for mild stinging sensation and redness in the left eye for almost 1-year and a half.

    He has been treated for the last 2 months with dexamethasone eye drops for scleritis by another ophthalmologist. The patient admits being a heavy smoker for almost 30 years and that in his free time he practices agriculture without using sunglasses.

    All other ophthalmological findings were normal.

    These features are biomarkers with prognostic and predictive value, which provide a characterization of tumor phenotypes in a non-invasive manner. The clinical application of radiomics is hampered by challenges such as lack of image acquisition and analysis standardization.

    Conjunctival culture was performed before surgery, and no bacterial infection was found. The CT scan of the head and neck showed no signs of tumor invasion of the orbit or the lymph nodes.

    skin papillomas pictures

    The limbic lesion was removed surgically with 2 mm margin of hpv 16 positive head and neck cancer tissue and diathermy of the adjacent sclera was done. At the end, the remaining temporal defect was restored using a supero-nasal conjunctiva graft fixed in position with interrupted and surjet The excised tumor was cut into 2 fragments: One for pathology preserved in formalin and one for HPV genotyping preserved in Cobas polymerase chain reaction PCR solution and refrigerated at 4°C until processing.

    hpv 16 positive head and neck cancer

    Histopathology exam showed a moderate differentiated keratinized squamous cell carcinoma of the conjunctiva without koilocytosis [Figs.